Part:BBa_K3747116

Periplasmic nitrate reductase H (napH) from Paracoccus denitrificans DSM 413

This gene is part of the nap operon from P. denitrificans DSM 413. Nap is the first enzyme of the denitrification pathway, converting nitrate (NO₃^-) into nitrite (NO₂^-). NapH encodes a putative ferredoxin. NapH can oxidize quinones and subsequently transfer the generated electrons to NapG. NapG redirects them to NapC, which transfers them to the catalytic subunit NapA [1].

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 801
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 801
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 801
  Illegal BamHI site found at 200
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 801
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 801
  Illegal NgoMIV site found at 25
  Illegal NgoMIV site found at 231
  Illegal NgoMIV site found at 251
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1] Yang, J., Feng, L., Pi, S., Cui, D., Ma, F., Zhao, H. ping, & Li, A. (2020). A critical review of aerobic denitrification: Insights into the intracellular electron transfer. Science of the Total Environment, 731, 139080. https://doi.org/10.1016/j.scitotenv.2020.139080